Superior Effect of the Combination of Carbon-Ion Beam Irradiation and 5-Fluorouracil on Colorectal Cancer Stem Cells in vitro and in vivo.
作者： Woong Sub Koom , Sei Sai , Masao Suzuki , Akira Fujimori , Shigeru Yamada
DOI: 10.2147/OTT.S276035
关键词:
摘要: Background The aim of this study was to investigate whether carbon-ion beam irradiation in combination with 5-fluorouracil (5-FU) is superior alone targeting colorectal cancer stem-like cells (CSCs). Materials and Methods Human (CRC) cells, HCT116 HT29, were treated or 5-FU. Cell viability assay, colony spheroid formation apoptotic quantitative real-time PCR analysis apoptosis- autophagy-related gene expression performed. Results Carbon-ion dose-dependently decreased CRC cell showed significantly enhanced killing effect when combined 5-FU increased the percentage cells. some genes such as Bax, Bcl2, Beclin1 ATG7 induced by further forming capacity CD133+ subpopulations inhibited Histopathologically, destroyed more xenograft tumor resulted necrosis, cavitation, fibrosis, compared alone. Conclusion In conclusion, treatment has potential kill including CSCs inducing apoptosis autophagy.
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