The consequences of stepwise deletions from the signal-processing site of beta-lactamase.

作者: A. Plückthun , J.R. Knowles

DOI: 10.1016/S0021-9258(18)61294-3

关键词:

摘要: Amino acids have been deleted from the processing site of pre-beta-lactamase, either into signal sequence or mature protein. Whereas loss more than 2 amino acid residues C-terminal end prevents translocation protein periplasm, removal two beginning has no effect on truncated The insertion an additional one to three at detectable phenotypic consequence either. It appears that many sequences for first few allow successful and processing. In sharp contrast, (but not both) flank results in a severe growth defect host cell very low expression Yet side site, both flanking normal secretion cleavage. These illustrate limits around junction suggest interference with cleavage step can lead only aborted but also pleiotropic consequences organism.

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