Loss of DNA methylation and histone H4 lysine 20 trimethylation in human breast cancer cells is associated with aberrant expression of DNA methyltransferase 1, Suv4-20h2 histone methyltransferase and methyl-binding proteins.

摘要: Cancer cells are characterized by epigenetic dysregulation, including global genome hypomethylation, regional hypo- and hypermethylation, altered histone modifications, disturbed genomic imprinting. Despite the long-established fact that DNA hypomethylation is a common feature of tumors, very little known about evolution this other alterations during tumor progression. The present study was undertaken to characterize status dysregulation in three human breast cancer cell lines (MCF-7, MDA-MB-231 MDA-MB-231(S30) represent different stages cancer. Our data show significant cellular compared non- tumorigenic MCF-10-2A epithelial cells. Interestingly, more malignant MDA-MB- 231 have prominent loss methylation accompanied expression maintenance methyltransferase DNMT1, methyl-binding proteins MeCP2 MBD2, decreased trimethylation lysine 20 H4 hyperacetylation MCF-7 decrease diminished Suv4-20h2 methyltransferase. results demonstrate extensive epigenenic than MCF-7. These which associated with increased properties Such may contribute be indicative formation aggressive phenotype