Three-dimensional bone marrow cell and tissue culture system
作者： Brian A. Naughton , Gail K. Naughton
DOI:
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摘要: The present invention relates to a three-dimensional cell culture system which can be used variety of different cells and tissues in vitro for prolonged periods time. In accordance with the invention, derived from desired tissue are inoculated grown on pre-established stromal support matrix. matrix comprises cells, such as fibroblasts actively growing Stromal may also include other found loose connective endothelial macrophages/monocytes, adipocytes, pericytes, reticular bone marrow stroma, etc. provides support, growth factors, regulatory factors necessary sustain long-term active proliferation culture. When this system, proliferating mature segregate properly form components adult analogous counterparts vivo.
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