His-Tag-Mediated Dimerization of Chemoreceptors Leads to Assembly of Functional Nanoarrays

摘要: Transmembrane chemotaxis receptors are found in bacteria extended hexagonal arrays stabilized by the membrane and cytosolic binding partners, kinase CheA coupling protein CheW. Models of array architecture assembly propose cluster into trimers dimers that associate with one dimer two CheW monomers to form minimal “core unit” necessary for signal transduction. Reconstructing vitro chemoreceptor ternary complexes homogeneous functional exhibit native remains a challenge. Here we report His-tag-mediated receptor dimerization divalent metals is sufficient drive nativelike cytoplasmic fragment. Our results indicate initiates precedes formation partial activity. Restoration maximal activity coincides shift larger complexes, suggesting depends on interactions beyond cor...