Simple and highly efficient method for transient in vivo gene transfer to mid-late pregnant mouse uterus
作者： Shinsuke Koyama , Tadashi Kimura , Kazuhide Ogita , Hitomi Nakamura , Chisa Tabata
DOI: 10.1016/J.JRI.2005.12.007
关键词:
摘要: Abstract Up- and down-regulation of various genes in the placenta, decidua amnion has been reported during mid-late period pregnancy pregnancy-related complications, such as preeclampsia preterm labor. However, whether this gene regulation at feto-maternal interface directly influences physiology/pathophysiology disease remains unknown. In order to study problem, transient transfer into pregnant uterus term would be a useful tool. We injected exogenous plasmid entrapped using commercially available Hemagglutinating Virus Japan Envelope (HVJ-E) vector system (GenomONE Neo, Ishihara Sangyo) extra-amniotic space upper part mouse on day 14.5 post-coitus (p.c.). Luciferase activity driven by cytomegalovirus promoter was detectable for 3 days after transfection upper, middle lower uterus. β-Galactsidase localized basal lamina decidual membrane fetal membrane. Exogenous not transmitted fetus. The course disturbed procedure; rupture membranes, intrauterine growth restriction birth were observed. Thus, we demonstrated that method is highly efficient minimally invasive, expect procedure will tool analyze pathophysiology disorders.
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