In-vivo evaluation of the permeability of the blood-brain barrier to arsenicals, molybdate, and methylmercury by use of online microdialysis-packed minicolumn-inductively coupled plasma mass spectrometry.

摘要: To study the permeability of blood–brain barrier (BBB) to arsenates, arsenite, monomethylarsonic acid (MMA), dimethylarsinic (DMA), molybdate, and methylmercury, transfer behavior these species, we constructed an automatic online analytical system comprising a microdialysis sampling device, minicolumn packed with nonfunctionalized poly(vinyl chloride) beads, inductively coupled plasma mass spectrometer for continuous in-vivo measurement their dynamic variation in extracellular space brains living rats. By using ion–polymer interactions as novel working mechanism sample pretreatment volume-limited microdialysate, simplified operating procedure conventional solid-phase extraction reduced contribution blank chemicals used. After optimizing this hyphenated system, measured its performance by analysis NIST standard reference materials 1640a (trace elements natural water) 2672a human urine) monitoring compounds tested fluid (ECF) rat brain. We found that intraperitoneal administration led observable BBB DMA, MMA, molybdate. Nevertheless, limited sensitivity size samples meant detection MeHg ECF remained problematic, even when administered dose 20 mg kg–1 body weight. On basis practical demonstrations, suggest our could be used not only kinetics four arsenicals molybdate brain but also describe associated neurotoxicity terms exposure toxic metals species.