Effect of foscarnet induction treatment on quantitation of human cytomegalovirus (HCMV) DNA in peripheral blood polymorphonuclear leukocytes and aqueous humor of AIDS patients with HCMV retinitis. The Italian Foscarnet Study Group.

摘要: The aim of this study was to investigate peripheral blood polymorphonuclear leukocytes and, whenever possible, aqueous humor from 65 AIDS patients with ophthalmoscopically diagnosed human cytomegalovirus (HCMV) retinitis determine (i) whether consistently carry viral DNA and (ii) what extent foscarnet induction treatment decreases levels. HCMV quantified by PCR using densitometric analysis hybridization products obtained external standards a standard curve which the number genome equivalents test samples, normalized an internal amplification control, interpolated. Results showed that 56 (86.1%) were positive for prior treatment. Of 41 (73.2%) whose had become negative after induction, only 5 high load (> 5,000 per 2 x 10(5) leukocytes) whereas as many 13 15 (26.8%) remaining induction. Finally, nine (13.8%) DNA-negative treatment, three shifted ganciclovir, while six erroneously enrolled in study. Pre- postinduction samples 12 patients; all these 4 became negative, 6 marked decrease DNA, nearly stable low In conclusion, is valuable tool etiologic diagnosis monitoring patients. present retinitis. Foscarnet highly effective suppressing or reducing levels both humor. Images