Neurotrophins, Depolarization and Second Messengers Interact to Control Serotonergic Neuronal Differentiation

摘要: Publisher Summary The serotonergic system plays a crucial role in the development of central nervous as well sleep-wake-arousal state adult mammal. Altered levels serotonin (5-HT) have been implicated major affective, anxiety, impulse, and personality disorders, schizophrenia Alzheimer's disease. However, epigenetic factors that control differentiation, survival, plasticity neurons are at present not definitively characterized. A better understanding mechanisms regulate 5-HT metabolism might facilitate manipulation endogenous vivo to alter course these various neurological disorders. This chapter discusses morphology proliferating differentiated RN46A cells expression neurofilament proteins cells. Neurofilament is developmentally regulated, increased NF-L NF-M were observed when grown 39°C. Increasing intracellular cAMP resulted robust up-regulation both NF-M. promoters do contain cAMP-response elements. These data suggest secondarily cAMP-dependent protein kinase (PKA) activity enhanced expression. little NF-C was detected under any experimental conditions, suggesting activation PKA insufficient initiate mature pattern NF triplet consistent with results demonstrated occurs subsequent synaptogenesis, probably response an unidentified target-derived signal.