Keratin 8 is a novel autoantigen of rheumatoid arthritis.
作者： Xiaoxu Wang , Peng Chen , Jiawen Cui , Chunhe Yang , Hongwu Du
DOI: 10.1016/J.BBRC.2015.07.161
关键词:
摘要: Abstract Objective This research aims to verify Keratin 8 (K8) as a specific autoantigen in rheumatoid arthritis (RA). Methods First, total RNA was extracted from HaCaT cell obtain cDNA by inverse transcription. Then, PCR performed amplify corresponding gene K8 primers. Next, cloning, expression, and purification technology were used the recombinant human (rhK8). At last, purified rhK8, after identified mass spectrometer, perform further disease-related Western blotting ELISA test with real clinical samples. Results Purified rhK8 successfully obtained then confirmed antigenicity of arthritis. The reactivity serum IgG against detected 34 50 RA patients (68%). antibodies significantly higher than healthy controls systemic lupus erythematosus (SLE) patients. Conclusion novel RA.
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