Evaluation of cytotoxicity, biophysics and biomechanics of cells treated with functionalized hybrid nanomaterials

摘要: Hybrids consisting of carboxylated, single-walled carbon nanotube (c-SWNT)–silver nanoparticles (AgNPs)-DNA–poly vinyl alcohol (PVA) are synthesized via sequential functionalization to mimic the theragnostic (therapy and diagnosis) system. Carboxylation SWNT has minimized metal impurities with plenty –COOH groups produce hybrid (c-SWNT-AgNPs). The is further wrapped DNA (hybrid-DNA) encapsulated PVA as composite (HC). Materials were tested against human alveolar epithelial cells (A549), mouse fibroblasts (NIH3T3) bone marrow stromal (HS-5). composition-sensitive physico-chemical interactions, biophysics biomechanics materials-treated evaluated. cell viability was improved for HC, hybrid-PVA c-SWNT when compared hybrid. showed less than 60% at high dose (40 µg ml−1) HC retained 80% or more viability. treatment nanomaterials considerably changed morphology intercellular interaction respect composition materials. Peculiarly, PVA-coated found minimize growth invadopodia A549 cells, which responsible proliferation cancer cells. Surface roughness increased after hybrid, where cytoplasmic regions specifically higher roughness. Nanoindentation results suggest that changes in occurred owing possible internalization force spectra treated indicated a greater between distinct stiffness demonstrated surface adherence on inside