Interleukin-12 inhibits tumor growth in a novel angiogenesis canine hemangiosarcoma xenograft model.

摘要: We established a canine hemangiosarcoma cell line derived from malignant endothelial cells comprising spontaneous tumor in dog to provide renewable source of for studies angiogenesis malignancy. Pieces the biopsy were engrafted subcutaneously bg/nu/XID mouse allowing expand vivo. A line, SB-HSA, was xenograft. SB-HSA expressed vascular growth factor (VEGF) receptors 1 and 2, CD31, CD146, αvβ3 integrin, produced several factors cytokines, including VEGF, basic fibroblast factor, interleukin (IL)-8 that are stimulatory growth. These results indicated recapitulated features mitotically activated endothelia. In vivo, stimulated robust angiogenic responses mice formed masses composed aberrant channels immunocompromised providing novel opportunities investigating effectiveness antiangiogenic agents. Using this model, we determined IL-12, cytokine with both immunostimulatory effects, suppressed induced by, of, cells. The model have described offers unique pursue further investigations as well other approaches cancer therapy.