In vitro and in vivo identification of metabolites of magnoflorine by LC LTQ‐Orbitrap MS and its potential pharmacokinetic interaction in Coptidis Rhizoma decoction in rat

摘要: Magnoflorine, an important aporphine alkaloid in Coptidis Rhizoma, is increasingly attracting research attention because of its pharmacological activities. The vivo and vitro metabolism magnoflorine was investigated by LC LTQ-Orbitrap MS. In samples including rat urine, feces, plasma bile were collected separately after both oral (50 mg kg−1) intravenous administration (10 mg kg−1) magnoflorine, along with prepared incubating intestinal flora liver microsome. As a result, 12 metabolites found biological samples. Phase I identified all samples, while phase II mainly detected bile. pharmacokinetic study, rats not only dosed via (15, 30 60 mg kg−1) but also Rhizoma decoction (equivalent to 30 mg kg−1 magnoflorine) intragastric investigate the interaction rest compounds Rhizoma. Studies showed that possessed lower bioavailability faster absorption elimination. However, parameters altered significantly (p < 0.05) when administered decoction. Oral gavage decreased elimination rates which revealed there existed interactions between ingredients Copyright © 2015 John Wiley & Sons, Ltd.