IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1

摘要: Sensitization to cockroach allergens is associated with the development of asthma. Bla g 1 a German allergen that shows allergenic cross-reactivity American allergen, Per 1, and has molecular structure composed multiple tandem amino-acid repeats. Two consecutive repeats are not identical but form duplex constitutes basic unit 1. By mass, purified natural would contain approximately two duplexes. We investigated pattern IgE antibody binding this repeated structure, whether one or duplexes sufficient for binding. Recombinant (r)Bla were expressed in Escherichia coli Pichia pastoris, analyzed monoclonal by ELISA and/or immunoblotting. Optimal rBla expression was obtained using methanol-inducible P. pastoris (> 95% pure protein, yield 48 mg x L(-1)), produced as forms mass 43, 32, 21 6 kDa, result proteolytic cleavage. There an excellent correlation between recombinant (r = 0.91, n 29, P < 0.001), immunoblot analysis showed single The suitable structural studies candidate clinical use diagnosis allergy new immunotherapy.