Anti-inflammatory effect of lycopene in SW480 human colorectal cancer cells.

摘要: BACKGROUND/OBJECTIVES: Although the antioxidative effects of lycopene are generally known, molecular mechanisms underlying anti-inflammatory properties not fully elucidated. This study aimed to examine role and mechanism as an inhibitor inflammation. METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, 30 μM lycopene. The MTT assay was performed determine on cell proliferation. Western blotting observe expression inflammation-related proteins, including nuclear factor-kappa B (NF-κB), kappa (IκB), mitogen-activated protein kinase (MAPK), extracellular signal-related (ERK), c-jun NH2-terminal (JNK), p38 (p38 MAP kinase). Real-time polymerase chain reaction investigate mRNA tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2). Concentrations (NO) prostaglandin E₂ (PGE₂) determined via enzyme-linked immunosorbent assays. RESULTS: In LPS, TNF-α, IL-1β, IL-6, iNOS, COX-2 decreased significantly in a dose-dependent manner (P CONCLUSIONS: Lycopene restrains NF-κB JNK activation, which causes inflammation, suppresses COX-2, iNOS SW480 cells.