Electron microscopic and biochemical study of lipoprotein synthesis in the isolated perfused rat liver.

摘要: The isolated perfused rat liver was used to study the 300-800 A electron-opaque bodies which had previously been described in cell Golgi apparatus, smooth endoplasmic reticulum, and space of Disse. When perfusion medium enriched with linoleate, number electron opacity these particles increased markedly. Sequential biopsies showed that they appeared first surfaced terminal ends rough reticulum proper, apparatus later After 60 min perfusion, same size shape as those cells could be large numbers from d Puromycin markedly depressed production by livers an oleate-rich medium; however, it did not interfere formation cytoplasmic droplets neutral fat. In keeping findings, puromycin blocked incorporation oleate-14C into lipoprotein triglyceride perfusate, but appearance labeled fatty acid tissue triglyceride. also leucine-3H both protein perfusate lipoprotein. We concluded observed are, all likelihood, very low density lipoproteins their is puromycin, presumably through interference synthesis apoprotein.