State-of-the-art in fast liquid chromatography-mass spectrometry for bio-analytical applications.

摘要: There is an increasing need of new bio-analytical methodologies with enough sensitivity, robustness and resolution to cope the analysis a large number analytes in complex matrices short time. For this purpose, all steps included any method (sampling, extraction, clean-up, chromatographic detection) must be taken into account achieve good reliable results cost-effective methodologies. The purpose review describe state-of-the-art most employed technologies period 2009-2012 fast liquid chromatography coupled mass spectrometry (LC-MS) for applications. Current trends involve use several column will focus on two frequently applied: sub-2μm particle size packed columns ultra high pressure (UHPLC) separations porous-shell attain efficiency reduced back-pressures. Additionally, recent automated sample extraction clean-up reduce manipulation, variability total time applications such as on-line solid phase HPLC or UHPLC methods, other approaches molecularly imprinted polymers, restricted access materials, turbulent flow also addressed. even ultra-high manipulation solve ion suppression enhancement matrix effects presented. advantages drawbacks these sensitive biological samples are going discussed by means relevant