Effects of chito-oligosaccharide on piglet jejunal explants: an histological approach

摘要: Abstract Antibiotics have been widely used in piglet diets to promote growth performance and reduce diarrhea incidence. However, the resistance of pathogens antibiotics risk residues animal products induced a growing interest use alternatives in-feed antibiotics. Chito-oligosaccharide (COS), natural alkaline polymer glucosamine is currently being tested as substitute for In weaned piglets, COS has positive effects on promoting growth, which may be related its action intestinal morphology, immune ability beneficial microbiota. previous studies shown variable results with effective doses ranging from 30 mg/kg 5 g/kg. Therefore, goal this study was test hypothesis that can an alternative by improve morphology using jejunal explant model. The explants were exposed 4 h following treatments: control – only culture media 0.025 mg/ml, 0.05 0.1 mg/ml 0.15 mg/ml. After incubation period processed histological morphometrical analysis. changes evaluated adapted score based intensity severity lesions. Mild observed different treatments; however, no significant difference score, villi height, crypt depth or villus : ratio between COS-groups control. addition, goblet cells density remained statistically similar group. Our indicate exposure levels effect pig’s explants. research will provide guidance low dosage supplementation weaning pigs.