Model of experimental chronic osteomyelitis in rats

摘要: We describe here a Sprague-Dawley rat model for chronic osteomyelitis. Staphylococcus aureus and sodium morrhuate were implanted by either microdrilling or direct needle injection into the tibiae of rats. Of 107 rats, 87 (81%) developed osteomyelitis when high-speed drill was used implantation, 27 (51%) 53 rats inoculation (chi square = 9.81, P less than 0.01). Demonstrated histopathological changes included presence resorption bays filled with osteoclasts. Quantitative microbiological monitoring tibial count confirmed disease chronicity, yielding stable numbers CFU (10(6.29 +/- 0.27) ) S. over 70 days. Infected animals became anemic lost weight. The erythrocyte sedimentation rates leukocyte counts not elevated. Roentgenograms provided best correlation number organisms in infected (r2 0.80). Rats treated oxacillin (120 mg/kg per day) ceftriaxone (50 day). Treatment 14 28 days reduced but did reliably sterilize bones, suggesting that this resistant to prolonged antimicrobial therapy.