Agar disk elution method for susceptibility testing of Mycobacterium marinum and Mycobacterium fortuitum complex to sulfonamides and antibiotics.

摘要: An agar disk elution method using round well plates, supplemented Mueller-Hinton agar, and commercial drug disks is described for susceptibility testing of Mycobacterium marinum the rapidly growing mycobacteria to antibiotics sulfonamides. By this method, 14 strains M. were susceptible rifampin, doxycycline, minocycline, trimethoprim-sulfamethoxazole. Identical results obtained with Middlebrook 7H10 drugs prepared from standard powders. With 58 isolates fortuitum chelonei, had a 92% correlation broth minimal inhibitory concentration determinations cefoxitin greater than 98% kanamycin, amikacin, Sixty-nine percent chelonei amikacin on resistant 15 16 erythromycin in by when an endpoint no growth was used either agar. The offers practical most antibacterial agents against these mycobacterial species. Images