Inhibition of angiotensin-converting enzyme by captopril: A novel approach to reduce ischemia-reperfusion injury after lung transplantation

摘要: Abstract Objectives: Ischemia-reperfusion injury after lung transplantation involves the generation of free radicals. Captopril has been shown to be protective in models ischemia-reperfusion other organs by acting as a radical scavenger. The purpose this study was assess effects captopril against and evaluate ability scavenge radicals inhibit neutrophil activation an experimental model transplantation. Methods: A rat single-lung transplant used. Donor lungs were flushed preserved low-potassium dextran-glucose solution with (n = 5) without (500 μmol/L; n=5) for 18 hours at 4°C then transplanted reperfused 2 hours. At conclusion 2-hour reperfusion period, arterial blood gases, pressure, peak airway pressure measured. Lung tissue biopsy specimens obtained assessment wet/dry weight ratios, histology, sequestration (myeloperoxidase activity). Lipid peroxidation (F -isoprostane assay) analyzed from plasma samples lysates. Results: addition preservation significantly improved postreperfusion PO (312 ± 63.3 mm Hg vs 202 21.1 Hg; P =.006), (11.4 1.1 cm H O 15.6 1.5 O; =.001), ratio (4.9 0.4 15.8 10.9; =.008). Blood pressures did not differ between groups. No significant differences seen myeloperoxidase activity or F levels. Conclusions: use ameliorates extended cold period. mechanisms which is remain elusive but do appear include inhibition lipid peroxidation. This novel approach may lead function provide further insight into pathogenesis acute injury. (J Thorac Cardiovasc Surg 2000;120:573-80)