Proteomic profile of cultured human endothelial cells after exposition to simulated microgravity
作者： Daria N. Kashirina , Alexey S. Kononikhin , Andrey Yu Ratushnyy , Evgeny N. Nikolaev , Irina M. Larina
DOI: 10.1016/J.ACTAASTRO.2020.10.014
关键词:
摘要: Abstract The proteome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and simulated microgravity (sμG) using Random Positioning Machine (RPM) for 24 h was studied by chromatography-mass spectrometry. It revealed that the percentage ribosomal proteins involved in intercellular adhesion, regulation actin cytoskeleton, various pathways cell signaling mediated G-proteins, apoptosis ubiquitin-dependent protein catabolism increased sμG. At same time number associated with growth reduced. A significant increase peak intensities maintaining cytoskeleton stress fibers (myristoylated alanine-rich C-kinase substrate, filamin-A, alpha-actinin-1 myosin light polypeptide 6) response to unfolded (serpin H1, 78 kDa glucose-regulated protein, transitional endoplasmic reticulum ATPase) shown. time, a decrease intensity cofilin 1, which is capable breaking filaments, revealed. Thus, most pronounced effect on as well adhesion proteins, whose properties also depend structure cytoskeleton. prevent improper folding increases, translation apparatus rearranged
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