Flavonoids, flavonoid metabolites, and phenolic acids inhibit oxidative stress in the neuronal cell line HT-22 monitored by ECIS and MTT assay: a comparative study.

摘要: A real-time and label-free in vitro assay based on electric cell-substrate impedance sensing (ECIS) was established, validated, compared to an end-point MTT within experimental trial addressing the cytoprotective effects of 19 different flavonoids, flavonoid metabolites, phenolic acids their methyl esters HT-22 neuronal cell line, after induction oxidative stress with tert-butyl hydroperoxide. Among flavonoids under study, only those a catechol unit additional 4-keto group provided cytoprotection. The presence 2,3-double bond not structural prerequisite for neuroprotective effect. In case phenolics, substitution requirement activity. other phenolics ferulic acid or single hydroxy showed no Electrochemical characterization all compounds via square-wave voltammetry rather specific correlation between activity redox potential active but low molecular weight. Moreover this study used compare ECIS recordings results established assay. Whereas former provides time-resolved thus entirely unbiased information changes morphology that are unequivocally associated death, latter requires predefined exposure times strict causality metabolic death. However, assays standard lab equipment provide more economic way higher throughput.