Antitumor Immune Response and Interleukin 2 Production Induced in Colorectal Cancer Patients by Immunization with Human Monoclonal Anti-Idiotypic Antibody

摘要: Abstract The immunogenicity of human anti-idiotypic antibody has been investigated using a monoclonal (105AD7) which interacts with the binding site 791T/36, mouse against gp72 antigen. This antigen is frequently expressed in gastrointestinal cancer; therefore, six patients advanced colorectal cancer have immunized 105AD7 as an aluminum hydroxide gel precipitate phase I clinical study. Cryopreserved blood mononuclear cells were tested for vitro proliferative responses by [3H]thymidine incorporation; plasma samples enzyme-linked immunosorbent assay anti-anti-idiotypic and antitumor antibodies, interleukin 2. Proliferative to positive tumor seen four five tested; parallel most these patients. Interleukin 2 was detected after immunization, peak levels up 7 units/ml. No toxicity related anti-idiotype immunization no or anti-anti-idiotype antibodies seen. study shows that immunogenic patients, inducing cellular production. suggests may considerable potential immunotherapy cancer.