Mobilization of plasmid pHSV106 from Escherichia coli HB101 in a laboratory-scale waste treatment facility.

摘要: Abstract The mobilization of plasmid pHSV106 from Escherichia coli HB101, in a laboratory model waste treatment facility, by both and indigenous wastewater strains E. was monitored transfer antibiotic resistance characteristics detection DNA sequences recipient cells. The demonstrated to occur under several different conditions, such as media composition, microbial concentrations, flow rates. herpes simplex virus thymidine kinase gene used hybridization marker confirm the occurrence transfer. use HB101 (recA mutant) host cell implies that recA functions are unnecessary for