Profiling of RNA Structure at Single-Nucleotide Resolution Using nextPARS.
作者： Uciel Chorostecki , Toni Gabaldon , Ester Saus , Jesse R Willis
DOI: 10.1007/978-1-0716-1307-8_4
关键词:
摘要: RNA molecules play important roles in almost every cellular process, and their functions are mediated by sequence structure. Determining the secondary structure of RNAs is central to understanding function evolution. probing techniques coupled high-throughput sequencing allow determining structural features at transcriptome-wide scales. Our group recently developed a novel Illumina-based implementation vitro parallel structures called nextPARS.Here, we describe protocol for computation nextPARS scores use obtain profile (single- or double-stranded state) an single-nucleotide resolution.
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