Inhibition of cell differentiation and cell cohesion by tunicamycin in Dictyostelium discoideum
作者： Tak-Yee Lam , Chi-Hung Siu
DOI: 10.1016/0012-1606(82)90185-3
关键词:
摘要: Abstract The effects of tunicamycin on protein glycosylation and cell differentiation were examined during early development Dictyostelium discoideum. Tunicamycin inhibited growth reversibly in liquid medium. At a concentration 3 μg/ml, completely morphogenesis developing cells. These cells remained as smooth lawn failed to undergo chemotactic migration. expression EDTA-resistant contact sites was also inhibited. inhibition by reversible if washed free the drug within first 10 hr incubation. After 12 development, protected from sheath. When treated with incorporation [3H]mannose [3H] fucose approximately 75% 45 min while no significant [3H]leucine observed initial treatment. further evidenced reduction number glycoproteins “stained” 125I-labelled con A. A developmentally regulated high-molecular-weight glycoproteins, including site glycoprotein (gp80), undetectable when labelled [3H]fucose presence tunicamycin. It is therefore evident that N-glycosidically linked carbohydrate moieties may play crucial role intercellular cohesiveness D.
elsevier.com
sci-hub.se 参考文章(44)
T.Y. LAM, G. PICKERING, J. GELTOSKY, C.H. SIU, Differential Cell Cohesiveness Expressed by Prespore and Prestalk Cells of Dictyostelium discoideum. Differentiation. ,vol. 20, pp. 22- 28 ,(1981) , 10.1111/J.1432-0436.1981.TB01151.X
S. D. Rosen, J. A. Kafka, D. L. Simpson, S. H. Barondes, Developmentally regulated, carbohydrate-binding protein in Dictyostelium discoideum. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 70, pp. 2554- 2557 ,(1973) , 10.1073/PNAS.70.9.2554
Chi-Hung Siu, Richard A. Lerner, William F. Loomis, Rapid accumulation and disappearance of plasma membrane proteins during development of wild-type and mutant strains of Dictyostelium discoideum. Journal of Molecular Biology. ,vol. 116, pp. 469- 488 ,(1977) , 10.1016/0022-2836(77)90080-8
D. Duksin, P. Bornstein, Changes in surface properties of normal and transformed cells caused by tunicamycin, an inhibitor of protein glycosylation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 74, pp. 3433- 3437 ,(1977) , 10.1073/PNAS.74.8.3433
Frederick T. Marin, Michele Goyette-Boulay, Frank G. Rothman, Regulation of development in Dictyostelium discoideum. III. Carbohydrate-specific intercellular interactions in early development. Developmental Biology. ,vol. 80, pp. 301- 312 ,(1980) , 10.1016/0012-1606(80)90406-6
Tak Yee Lam, Chi-Hung Siu, Synthesis of stage-specific glycoproteins in Dictyostelium discoideum during development Developmental Biology. ,vol. 83, pp. 127- 137 ,(1981) , 10.1016/S0012-1606(81)80015-2
Chi-Hung Siu, Richard A. Lerner, Gloria Ma, Richard A. Firtel, William F. Loomis, Developmentally regulated proteins of the plasma membrane of Dictyostelium discoideum. The carbohydrate-binding protein. Journal of Molecular Biology. ,vol. 100, pp. 157- 178 ,(1976) , 10.1016/S0022-2836(76)80146-5
William M. BONNER, Ronald A. LASKEY, A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels FEBS Journal. ,vol. 46, pp. 83- 88 ,(1974) , 10.1111/J.1432-1033.1974.TB03599.X
L. Lehle, W. Tanner, The specific site of tunicamycin inhibition in the formation of dolichol-bound N
-acetylglucosamine derivatives FEBS Letters. ,vol. 71, pp. 167- 170 ,(1976) , 10.1016/0014-5793(76)80922-2
M. Azim, H. Surani, Glycoprotein synthesis and inhibition of glycosylation by tunicamycin in preimplantation mouse embryos: Compaction and trophoblast adhesion Cell. ,vol. 18, pp. 217- 227 ,(1979) , 10.1016/0092-8674(79)90370-2