Glycosylphosphatidylinositol-modified murine B7-1 and B7-2 retain costimulator function.

摘要: Glycosylphosphatidylinositol (GPI)-modified variants of murine B7-1 and B7-2 cell surface costimulators were produced via chimerization with alternative GPI-modification signal sequences from decay-accelerating factor (DAF). GPI anchorage was verified by demonstrating phosphatidylinositol-specific phospholipase C (PI-PLC) sensitivity the chimeric polypeptides in both immunofluorescence/flow-cytometric immunoprecipitation analyses. The various GPI-modified B7-1:DAF B7-2:DAF shown to retain costimulator function, an vitro proliferation assay vivo triggering cytotoxicity assay. findings indicate that function for is not dependent upon native hydrophobic transmembrane anchorage. Moreover, functionality enhancing immunogenicity T lymphoma line EL-4 suggests a novel route generating APC-centered immunotherapeutics, including cellular cancer vaccines, based protein transfer costimulators.