Polyamide 6.6 thin films with distinct ratios of the main chemical groups: Influence in the primary neuronal cell culture

摘要: Abstract Neuronal cell cultures have been instrumental to the advance of our knowledge in a wide range areas. One limitations this model is lack stability, over time, substrate materials that are used neuronal cultures. In work, sputtering was permanently modify glass coverslips with polyamide 6.6 thin film, using different discharge gases. Differences chemical composition films, resulting from conditions deposition, were correlated surface properties. After characterization by SEM, TEM, AFM, XPS, zeta-potential, wettability and evaluation mechanical properties, some surfaces selected for vitro testing development cultured cerebrocortical neurons. The results showed charge particularly affected variation deposition parameters, although wettability, example, did not present significant variations. tests revealed higher NH2/COOH ratio allowed adhesion differentiation neurons, morphology similar obtained after plating cells on poly- d -lysine-coated glass. This work opens new perspectives permanent modification towards multifunctional neural compatible open areas bioengineering applications.