Molecular basis of sex and reproductive status in breeding zebrafish
作者： C. R. Tyler , E. M. Santos , V. L. Workman , G. C. Paull , A. L. Filby
DOI: 10.1152/PHYSIOLGENOMICS.00284.2006
关键词:
摘要: The zebrafish (Danio rerio) is used extensively as a model species for studies on vertebrate development and assessing chemical effects reproduction. Despite this, the molecular mechanisms controlling reproduction are poorly understood. We analyzed transcriptomic profiles of gonads individual zebrafish, using 17k oligonucleotide microarray, to define basis sex reproductive status in sexually mature fish. gonadal transcriptome differed substantially between sexes. Among genes overexpressed females, 11 biological processes were overrepresented including mitochondrion organization biogenesis, cell growth and/or maintenance. males, six protein biosynthesis metabolism. Analysis expression gene families known be involved identified number differentially expressed ovaries testes sox belonging insulin-like factor activin-inhibin pathways. Real-time quantitative PCR confirmed nine most indicated that many transcripts likely switched off one sexes adult Significant differences seen gonad transcriptomes reproductively active females reflecting their stage maturation, whereas testis remarkably similar individuals. In summary, we have associated with (gonadal) specificity breeding established strong relationship ovarian females.
manchester.ac.uk参考文章(56)
Josef V. Silha, Liam J. Murphy, Insulin-like growth factor binding proteins in development Advances in Experimental Medicine and Biology. ,vol. 567, pp. 55- 89 ,(2005) , 10.1007/0-387-26274-1_3
Jose Luis Trejo, Eva Carro, Deborah J. Burks, Experimental Models for Understanding the Role of Insulin-like Growth Factor-I and Its Receptor During Development Advances in Experimental Medicine and Biology. ,vol. 567, pp. 27- 53 ,(2005) , 10.1007/0-387-26274-1_2
Randy Rasmussen, Quantification on the LightCycler Springer, Berlin, Heidelberg. pp. 21- 34 ,(2001) , 10.1007/978-3-642-59524-0_3
Antony W. Wood, Cunming Duan, Howard A. Bern, Insulin-Like Growth Factor Signaling in Fish International Review of Cytology. ,vol. 243, pp. 215- 285 ,(2005) , 10.1016/S0074-7696(05)43004-1
Marek Dudas, Vesa Kaartinen, Tgf-beta superfamily and mouse craniofacial development: interplay of morphogenetic proteins and receptor signaling controls normal formation of the face. Current Topics in Developmental Biology. ,vol. 66, pp. 65- 133 ,(2005) , 10.1016/S0070-2153(05)66003-6
Yoshitaka Nagahama, Michiyasu Yoshikuni, Masakane Yamashita, Toshinobu Tokumoto, Yoshinao Katsu, Regulation of oocyte growth and maturation in fish Current Topics in Developmental Biology. ,vol. 30, pp. 103- 145 ,(1995) , 10.1016/S0070-2153(08)60565-7
Alfonso Paredes, Cecilia Garcia-Rudaz, Bredford Kerr, Veronica Tapia, Gregory A. Dissen, Maria E. Costa, Anda Cornea, Sergio R. Ojeda, Loss of synaptonemal complex protein-1, a synaptonemal complex protein, contributes to the initiation of follicular assembly in the developing rat ovary. Endocrinology. ,vol. 146, pp. 5267- 5277 ,(2005) , 10.1210/EN.2005-0965
M. Blázquez, P.T. Bosma, E.J. Fraser, K.J.W. Van Look, V.L. Trudeau, FISH AS MODELS FOR THE NEUROENDOCRINE REGULATION OF REPRODUCTION AND GROWTH Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. ,vol. 119, pp. 345- 364 ,(1998) , 10.1016/S0742-8413(98)00023-1
Renae L. Malek, Hedieh Sajadi, Joseph Abraham, Martin A. Grundy, Glenn S. Gerhard, The effects of temperature reduction on gene expression and oxidative stress in skeletal muscle from adult zebrafish. Comparative Biochemistry and Physiology C-toxicology & Pharmacology. ,vol. 138, pp. 363- 373 ,(2004) , 10.1016/J.CCA.2004.08.014
Chaoming Wen, Zhenhai Zhang, Weiping Ma, Min Xu, Zilong Wen, Jinrong Peng, Genome-wide identification of female-enriched genes in zebrafish. Developmental Dynamics. ,vol. 232, pp. 171- 179 ,(2005) , 10.1002/DVDY.20210