Detection of protein biomarkers using high-throughput immunoblotting following focal ischemic or penetrating ballistic-like brain injuries in rats.
作者： Changping Yao , Anthony J. Williams , Andrew K. Ottens , X.-C. May Lu , Renwu Chen
DOI: 10.1080/02699050802304706
关键词:
摘要: Primary objective: Recent efforts have been aimed at developing a panel of protein biomarkers for the diagnosis/prognosis neurological damage associated with acute brain injury.Methods and procedures: This study utilized high-throughput immunoblotting (HTPI) technology to compare changes between two animal models injury: penetrating ballistic-like injury (PBBI) which mimics created by gunshot wound transient middle cerebral artery occlusion (MCAo) is model stroke. Brain blood were collected 24-hours post-injury.Main outcomes results: identified in 18 proteins following PBBI 17 MCAo out total 998 screened proteins. Distinct differences observed models: five up- or down-regulated both models, 23 changed only one was differentially expressed. Western blots used verify HTPI results selected mea...
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