Pyrolysed 3D-Carbon Scaffolds Induce Spontaneous Differentiation of Human Neural Stem Cells and Facilitate Real-Time Dopamine Detection

摘要: Structurally patterned pyrolysed three-dimensional carbon scaffolds (p3D-carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed cell replacement therapy sensing released dopamine. In the absence factors (DF) material induces spontaneous hNSC into mature dopamine-producing neurons 3D-topography promotes neurite elongation. presence DF, approximate to 73-82% hNSCs obtain dopaminergic properties on carbon, a to-date unseen efficiency in both two-dimensional (2D) 3D environment. Due conductive environment, p3D-carbon serves as neurotransmitter trap, enabling electrochemical detection significantly larger dopamine fraction by derived than conventional 2D electrodes. This is first study its kind, presenting new that provide highly efficient phenotype combined with real-time situ confirmation fate hNSC-derived neurons.