Effect of 2-methoxyestradiol on SK-LMS-1 uterine leiomyosarcoma cells.

摘要: An endogenous metabolite of 17β-estradiol, 2-methoxyestradiol (2-ME), has affinity for estrogen receptors. This compound was reported to be a promising antitumor drug due its anti-proliferative effects on wide range tumor cell types. Numerous previous studies have been performed evaluate the cytotoxic 2-ME lines in following induction G2/M cycle arrest and subsequent apoptosis. Uterine leiomyosarcoma (ULMS) is relatively rare malignant smooth muscle that develops uterus layer. The aim present study examine vitro SK-LMS-1 human cells. MTT assay, terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling immunocytochemistry western blotting were performed. A high concentration (10-5 M) identified an effect Additionally, expression apoptosis markers upregulated presence 10-5 M 2-ME, according blot analysis. Furthermore, level autophagic marker, light chain 3, increased by treatment dose-dependent manner. associated with death induced upregulation phosphorylated extracellular-signal-regulated kinase 1/2 signaling pathway. results demonstrated which used as therapeutic agent treating solid tumors, exhibits apoptotic depending dose. Therefore, may potential reagent ULMS, but appropriate dose this should carefully selected.