Combination therapy profoundly improved skin flap survival by modulating KATP channels and nitric oxide

摘要: Abstract Purpose A potential therapeutic approach on skin flap necrosis is to target parallel pathways involved in necrosis. Azelaic Acid, Minoxidil and Caffeine combination was tried survival by their possible interaction with ATP sensitive potassium (KATP) channels nitric oxide pathway. Material methods Sprauge-Dawley rats were divided into 8 groups for surgery. acid, minoxidil, caffeine, or applied topically different groups. Two additional treated L-NAME glibenclamide addition the therapy. Percentage of calculated samples removed measure tissue malondialdehyde (MDA) (NO) expression inducible synthase (iNOS), Bcl-2 Bax proteins. Results Combination therapy profoundly decreased necrosis, MDA contents, pro-apoptotic protein (p  Conclusion Beneficial effect azelaic minoxidil caffeine rescuing from targeting signaling suggested applications clinical practice.