Functional genomics of intracellular peptide recognition domains with combinatorial biology methods.

摘要: Phage-displayed peptide libraries have been used to identify specific ligands for peptide-binding domains that mediate intracellular protein-protein interactions. These studies provided significant insights into the specificities of particular domains. For PDZ recognize C-terminal sequences, information has proven useful in identifying natural binding partners from genomic databases. SH3 internal proline-rich motifs, results database searches with phage-derived compared yeast-two-hybrid experiments produce overlap networks reliably predict In addition, phage-displayed and residues responsible ligand recognition, also engineer altered specificities.