Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 3. Short- and long-time dynamics of the excited-state proton transfer.
作者： Pavel Leiderman , Liat Genosar , Dan Huppert , Xiaokun Shu , S. James Remington
DOI: 10.1021/BI7009053
关键词:
摘要: Steady-state emission, femtosecond pump−probe spectroscopy, and time-correlated single-photon counting (TCSPC) measurements were used to study the photophysics excited-state proton transfer (ESPT) reactions in green fluorescent protein (GFP) variant S65T/H148D at three pH values: 6.0, 7.9, 9.5. Selective mutation of GFP caused a dramatic change steady-state behavior as compared wild-type (wt-GFP) studied earlier. An excitation wavelength dependence quantum yield strong emission band 510 nm (I* band) indicates competition between adiabatic non-adiabatic proton-transfer reactions. Pump−probe show that signal buildup probed is biphasic, where 0.8 amplitude ultrashort, <150 fs, 0.2 decreases with ∼10 ps time constant. This effect summary result ESPT carboxylate group Asp148 nonradiative processes. When lumi...
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