Direct Measurement of Long-Range Interaction Forces Between a Single Bacterium and a Substrate Using an Optical Trap

摘要: As discussed in Chapter 2, stable adhesion of a bacterium on surface requires the following events: transport to surface, attachment and subsequent resistance detachment. In general, long-range forces (including macromolecular bridging) govern probability cell whereas short-range strength ultimate adhesion. Brownian motion, convection or motility must over any energy barrier created by repulsive forces. The magnitude these need not be large repel particle from surface. We can roughly estimate force acting certain range that would create an insurmountable potential for as follows: crossing through fluctuations scales with factor, exp(−o max /kT),where k is Boltzmann’s constant (1.38×10−23 J/K), T absolute temperature (~300 K);19 therefore, 10 kT sufficient prevent particle. Assuming act around 50 nanometers, then only about one picoNewton (10−12 Newtons) required work.