One-week 96-well soft agar growth assay for cancer target validation.
作者： Ning Ke , Aaron Albers , Gisela Claassen , De-hua Yu , Jon E. Chatterton
DOI: 10.2144/04365ST07
关键词:
摘要: Soft agar growth, used to measure cell anchorage-independent proliferation potential, is one of the most important and commonly assays detect transformation. However, traditio...
future-science.com
unirioja.es
biotechniques.com 参考文章(14)
K Strebhardt, H J Stutte, H Rübsamen-Waigmann, J Yuan, A Hörlin, B Hock, Polo-like kinase, a novel marker for cellular proliferation. American Journal of Pathology. ,vol. 150, pp. 1165- 1172 ,(1997)
Nan Sook Lee, Taikoh Dohjima, Gerhard Bauer, Haitang Li, Ming-Jie Li, Ali Ehsani, Paul Salvaterra, John Rossi, Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells Nature Biotechnology. ,vol. 20, pp. 500- 505 ,(2002) , 10.1038/NBT0502-500
Sayda M. Elbashir, Jens Harborth, Winfried Lendeckel, Abdullah Yalcin, Klaus Weber, Thomas Tuschl, Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature. ,vol. 411, pp. 494- 498 ,(2001) , 10.1038/35078107
Daniel Horowitz, Andrew G King, COLORIMETRIC DETERMINATION OF INHIBITION OF HEMATOPOIETIC PROGENITOR CELLS IN SOFT AGAR Journal of Immunological Methods. ,vol. 244, pp. 49- 58 ,(2000) , 10.1016/S0022-1759(00)00253-2
Cynthia P. Paul, Paul D. Good, Ira Winer, David R. Engelke, Effective expression of small interfering RNA in human cells Nature Biotechnology. ,vol. 20, pp. 505- 508 ,(2002) , 10.1038/NBT0502-505
Erich A Nigg, Polo-like kinases: positive regulators of cell division from start to finish. Current Opinion in Cell Biology. ,vol. 10, pp. 776- 783 ,(1998) , 10.1016/S0955-0674(98)80121-X
Makoto Miyagishi, Kazunari Taira, U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells Nature Biotechnology. ,vol. 20, pp. 497- 500 ,(2002) , 10.1038/NBT0502-497
H. Fukazawa, S. Mizuno, Y. Uehara, A microplate assay for quantitation of anchorage-independent growth of transformed cells Analytical Biochemistry. ,vol. 228, pp. 83- 90 ,(1995) , 10.1006/ABIO.1995.1318
S. I. Shin, V. H. Freedman, R. Risser, R. Pollack, Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 72, pp. 4435- 4439 ,(1975) , 10.1073/PNAS.72.11.4435
Akhil Banerjea, Ming-Jie Li, Gerhard Bauer, Leila Remling, Nan-Sook Lee, John Rossi, Ramesh Akkina, Inhibition of HIV-1 by lentiviral vector-transduced siRNAs in T lymphocytes differentiated in SCID-hu mice and CD34+ progenitor cell-derived macrophages. Molecular Therapy. ,vol. 8, pp. 62- 71 ,(2003) , 10.1016/S1525-0016(03)00140-0