Simultaneous Analysis of Isoniazid and Its Impurities by CZE

作者: Adriana F. Faria , Juliana P. Vasconcelos , Raoni S. B. Gonçalves , Marcus V. N. de Souza , Marcone A. L. de Oliveira

DOI: 10.1007/S10337-012-2308-3

关键词:

摘要: An alternative methodology for simultaneous determination of isoniazid, isonicotinic acid, 4-cyanopyridine, ethyl isonicotinate and isonicotinamide by capillary zone electrophoresis, within an analysis time 11 min, is proposed. electrolyte composed 50 mmol L−1 acetic acid/sodium acetate buffer 12.5 Cu2+, analyte dilution in 1 Brij 35 Cu2+ +20 kV voltage were optimized. After evaluating some figures merit, such as linearity, precision, recovery quantification limit, the method was successfully applied to isoniazid tablets. The contents found 99.3 mg 0.3 corresponding % impurity regarding content active ingredient pharmaceutical formulation.

参考文章(7)
Hemant Bhutani, Saranjit Singh, Sanjay Vir, K.K. Bhutani, Raj Kumar, Asit K. Chakraborti, K.C. Jindal, LC and LC-MS study of stress decomposition behaviour of isoniazid and establishment of validated stability-indicating assay method Journal of Pharmaceutical and Biomedical Analysis. ,vol. 43, pp. 1213- 1220 ,(2007) , 10.1016/J.JPBA.2006.10.013
Adriana F. Faria, Marcus V.N. de Souza, Roy E. Bruns, Marcone A.L. de Oliveira, Simultaneous determination of first-line anti-tuberculosis drugs by capillary zone electrophoresis using direct UV detection. web science. ,vol. 82, pp. 333- 339 ,(2010) , 10.1016/J.TALANTA.2010.04.044
H. H. Fox, The Chemical Approach to the Control of Tuberculosis Science. ,vol. 116, pp. 129- 134 ,(1952) , 10.1126/SCIENCE.116.3006.129
Milan Štědrý, Michal Jaroš, Kateřina Včeláková, Bohuslav Gaš, Eigenmobilities in background electrolytes for capillary zone electrophoresis: II. Eigenpeaks in univalent weak electrolytes ELECTROPHORESIS. ,vol. 24, pp. 536- 547 ,(2003) , 10.1002/ELPS.200390061
Marcus Vinícius Nora de Souza, Marcelle de Lima Ferreira, Alessandra Campbell Pinheiro, Maurício Frota Saraiva, Mauro Vieira de Almeida, Marcelo Siqueira Valle, Synthesis and Biological Aspects of Mycolic Acids: An Important Target Against Mycobacterium tuberculosis The Scientific World Journal. ,vol. 8, pp. 720- 751 ,(2008) , 10.1100/TSW.2008.99
Floyd K, Dye C, Uplekar M, Global tuberculosis control: surveillance planning financing. WHO report 2008. Geneva Switzerland World Health Organization [WHO] 2008.. ,(2008)