Peanut lectin stimulates proliferation in colonic explants from patients with inflammatory bowel disease and colon polyps

摘要: Background/Aims: The TF antigen (galactose-β 1,3- N -acetylgalactosamine α) is overexpressed in malignant and premalignant colonic epithelium. Previous studies have shown that peanut lectin (PNA), which binds TF, mitogenic for normal human This study aimed to determine its effect on abnormal Methods: Crypt cell proliferation rate (CCPR) was measured using vincristine arrest mucus synthesis by incorporation of radiolabeled -acetyl glucosamine colonoscopic biopsy specimens cultured with without PNA. Results: Unstimulated CCPR greater patients ulcerative colitis than histologically colon. PNA (25 μg/mL) produced a 25% average increase tissues from colitis, Crohn's disease, polyps. In colitic incubated PNA, increased more double unstimulated controls, the response when adjacent were positive (avidin-biotin) histochemistry they negative. Mucus an 75% over 24 hours Conclusions: Increased expression epithelia may allow stimulation dietary galactose -acetylgalactosamine-binding lectins. If hyperplasia-dysplasia cancer hypothesis correct, this could explain colon risk colitis.