Use of superparamagnetic beads for the isolation of a peptide with specificity to cymbidium mosaic virus.
作者： Diana Jia Miin Ooi , Adriya Dzulkurnain , Rofina Yasmin Othman , Saw Hoon Lim , Jennifer Ann Harikrishna
DOI: 10.1016/J.JVIROMET.2006.05.001
关键词:
摘要: A modified method for the rapid isolation of specific ligands to whole virus particles is described. Biopanning against cymbidium mosaic was carried out with a commercial 12-mer random peptide display library. solution phase panning devised using streptavidin-coated superparamagnetic beads. The based more efficient than conventional immobilized target when viral as target. Enzyme-linked immunosorbent assay virus-binding peptides isolated from library identified seven affinity and one which had no significant binding odontoglossum ringspot virus. This should have broad application screening towards development diagnostic reagents without requirement cloning expression single antigens.
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sci-hub.se 参考文章(37)
D. D. Jensen, Mosaic or black streak disease of Cymbidium Orchids. Phytopathology. ,vol. 41, ,(1951)
S. S. Hong, P. Boulanger, Protein ligands of the human adenovirus type 2 outer capsid identified by biopanning of a phage-displayed peptide library on separate domains of wild-type and mutant penton capsomers. The EMBO Journal. ,vol. 14, pp. 4714- 4727 ,(1995) , 10.1002/J.1460-2075.1995.TB00153.X
Maxime Moulard, Mei-Yun Zhang, Dimiter S. Dimitrov, Novel Hiv Neutralizing Antibodies Selected from Phage Display Libraries Springer, Boston, MA. pp. 105- 117 ,(2004) , 10.1007/978-1-4419-8877-5_5
Carlos F. Barbas, Phage Display: A Laboratory Manual ,(2004)
William G.T. Willats, Phage display: practicalities and prospects. Plant Molecular Biology. ,vol. 50, pp. 837- 854 ,(2002) , 10.1023/A:1021215516430
Stephen J. Wylie, Michael G. K. Jones, Craig G. Webster, Diagnosis of plant viral pathogens Current Science. ,vol. 86, pp. 1- 4 ,(2004)
Clarence I. Kado, Virus diseases of Orchids. Circular. California Agricultural Experiment Station Extension Service. ,vol. 552, ,(1970) , 10.5962/BHL.TITLE.61071
John McCafferty, Kevin S. Johnson, CHAPTER 6 – Construction and Screening of Antibody Display Libraries Phage Display of Peptides and Proteins#R##N#A Laboratory Manual. pp. 79- 111 ,(1996) , 10.1016/B978-012402380-2/50008-3
B. Zhou, P. Wirsching, K. D. Janda, Human antibodies against spores of the genus Bacillus: A model study for detection of and protection against anthrax and the bioterrorist threat Proceedings of the National Academy of Sciences of the United States of America. ,vol. 99, pp. 5241- 5246 ,(2002) , 10.1073/PNAS.082121599
Angelika Ziegler, Lesley Torrance, Applications of recombinant antibodies in plant pathology. Molecular Plant Pathology. ,vol. 3, pp. 401- 407 ,(2002) , 10.1046/J.1364-3703.2002.00130.X