PAX2 suppresses apoptosis in renal collecting duct cells.

摘要: PAX2 is a transcription factor belonging to the evolutionarily conserved paired box family and required during development of central nervous system genitourinary axis. Mutations in gene cause rare autosomal dominant renal-coloboma syndrome, characterized by optic nerve colobomas renal hypoplasia. Recent analysis spontaneous mutant mouse model (1Neu) revealed that major hypoplasia reduced branching ureteric bud (UB) fewer nephrons. We have observed this abnormality associated with striking increase number UB cells undergoing programmed cell death nephrogenesis. To ascertain whether apoptosis directly linked level expression, we studied role cultured cells. show mIMCD-3 cells, murine collecting duct line high endogenous undergo when transfected anti-sense PAX2. In contrast, HEK293 expressing exogenous are protected against apoptotic induced caspase-2. has no effect on proliferation embryonic kidney or Our observations imply direct for survival