Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis

摘要: Identifying patients with germline MUTYH mutation-associated polyposis is presently difficult. The aim of this study to investigate the possibilities IHC as a screening test select for mutation analysis. expression protein in colorectal adenomas or cancer was studied by using three different (1 polyclonal and 2 monoclonal) antibodies six samples from biallelic mutations, single mutation, 11 without mutations. With antibody, carcinomas mutations showed strong supranuclear cytoplasmic staining epithelial nuclear staining. also observed nine out Samples incubated monoclonal non-specific pattern. Our results demonstrate that, contrast previous data, neoplastic cells does not discriminate mutated unmutated cases. At present, cannot be used clinical practice differentiate between tissue