Pressure Probe Technique for Measuring Water Relations of Cells in Higher Plants

摘要: A new method is described for continuously measuring cell turgor pressure (P), hydraulic conductivity (L p ), and volumetric elastic modulus (e) in higher plant cells, using a probe. This technique permits volume changes, ΔV, ΔP, to be determined with an accuracy of 10 −5 −6 μl 3 5·10 −2 bar, respectively. The main principle the same as probe developed by Zimmermann Steudle which transmitted transducer means oil-filled capillary introduced into cell. In order use small tissue effective compressible apparatus has sufficiently comparison itself. achieved accurately fixing oil/cell sap boundary very tip microcapillary electronic feedback mechanism, so that reduced about 2 10% volume. this way also, errors arising from compressibility temperature fluctuations can excluded. Measurements on tissues cells Capsicum annuum fruits yield e values 25 bar. Furthermore, shown function both volume; increases increasing larger cells.