Factors Controlling Sperm Entry into the Micropyles of Salmonid and Herring Eggs

摘要: When the micropyle area of salmonid (trout and salmon) eggs was observed continuously from moment insemination, spermatozoa were seen moving along surface chorion entering one by in a directed fashion. The ability to enter reduced after treatment chorions with pronase; this reduction sperm entry even before outer opening channel narrowed due gradual swelling pronase treatment. Herring spermatozoa, unlike most other marine fishes, motionless seawater. However, they became vigorously motile on contact herring egg entered rapidly efficiently. Motility initiation dependent extracellular calcium potassium. Sodium also appears be intricately involved process as demonstrated movement sodium-free treated acidic seawater, organic solvents, or glutaraldehyde, did not initiate area, observed. eggs. These observations suggest that areas possess some guidance factors which facilitate homologous into micropyle.