Characterizing invading glioma cells based on IDH1-R132H and Ki-67 immunofluorescence
作者： Hemragul Sabit , Mitsutoshi Nakada , Takuya Furuta , Takuya Watanabe , Yutaka Hayashi
DOI: 10.1007/S10014-013-0172-Y
关键词:
摘要: Glioma, the most common primary brain tumor, is characterized by proliferative-invasive growth. However, detailed biological characteristics of invading glioma cells remain to be elucidated. A monoclonal antibody (clone HMab-1) that specifically and sensitively recognizes isocitrate dehydrogenase-1 (IDH1) protein carrying R132H mutation can identify immunostaining. To investigate degree invasion in gliomas distinct grades proliferative capacity cells, immunofluorescent staining was conducted using antibodies against IDH1-R132H Ki-67 on 11 surgical autopsy specimens tumor core area. Higher numbers IDH1-R132H-positive area correlated with a higher grade. Double for demonstrated expressed were not stained antibody, ratio Ki-67-positive among significantly lower than all glioma. These data suggest grade have greater invasive potential possess low capacity.
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