Genetic and molecular characterization of Tn21, a multiple resistance transposon from R100.1.

摘要: Tge transposon Tn21 has been transposed from R100.1 to plasmid pACYC184 and, the resulting recombinants, R388. The sites of insertion and orientation element in several pACYC184::Tn21 recombinants have examined. Restriction enzyme analysis these resulted a detailed map Tn21; this is compared with published maps relevant part R100.1. Heteroduplex shown short inverted repeat sequences at ends element. With various vitro-generated deletion mutants Tn21, internal gene necessary for transposition (tnpA) was localized within terminal 4.3 kilobases right-hand end Genetic suggests that process proceeds via cointegrates. Since products are simple recipient replicon, must contain codes resolvase type activity (tnpR gene).