Synergistic Antitumor Activities of Docetaxel and Octreotide Associated with Apoptotic-Upregulation in Castration-Resistant Prostate Cancer

摘要: Androgen deprivation therapy has become the fist-line treatment of metastatic prostate cancer; however, progression to castrate resistance disease occurs in majority patients. Thus, there is an urgent need for improvements castration-resistant cancer. The aims present study were determine efficacy somatostatin analogue octreotide (OCT) combined with a low dose docetaxel (DTX) using castration resistant cancer cells and investigate involved molecular mechanisms vitro. anti-proliferative synergism potential effects determined by MTT assay. Induction apoptosis was analyzed employing annexing V propidium iodide staining flow cytometry. VEGFA, CASP9, CASP3 ABCB1 gene expression evaluated RT-PCR Q-RT-PCR analysis. OCT combination DTX treatments on DU145 cell migration also evaluated. Investigation revealed that administration had significant, synergistically greater cytotoxicity than or alone. two drugs caused more marked increase resulted suppression invasive either individual agent. There obvious caspase 3 alone two-drug groups, VEGFA markedly suppressed them. These results support conclusion analogues may enhance chemotherapy efficacies through multiple PCa line. This work provides preclinical rationale therapeutic strategies improve disease.