Enantioseparation of 1-phenyl-1-propanol on cellulose-derived chiral stationary phase by supercritical fluid chromatography II. Non-linear isotherm.

摘要: Abstract The separation of the enantiomers 1-phenyl-1-propanol by supercritical fluid chromatography on a chiral stationary phase, which consists cellulose tris (3,5-dimethylphenylcarbamate) coated silica support (Chiralcel-OD), is studied under overloaded, non-linear chromatographic conditions. Pulse experiments are performed at temperature 30  ° C using CO2 modified with methanol as mobile phase. parameters binary Langmuir adsorption isotherm determined inverse method, comparing experimental and simulated peak responses. Isotherm derived for modifier concentrations between 1 5% (w/w) operating pressures 125 185 bar, dependency parameters, namely Henry constant saturation capacity, conditions investigated.