Structures of the Euplotes crassusTec1 and Tec2 elements: identification of putative transposase coding regions

摘要: The Tec1 and Tec2 transposon-like element families of Euplotes crassus are highly unusual in that all 30,000 copies each family excised from the genome during a discrete time period macronuclear development. Complete nucleotide sequences were generated for Tec1-1 Tec2-1 elements, representing families. Open reading frames (ORFs) conserved position sequence between two although comprise one ORF (ORF2) split into overlapping ORFs (ORFs 2A 2B) Tec2-1. ORF1 Tec1-1, its homolog (ORF2B) contain TGA codons, which may be translated as Cys, observed other Euplotid genes. Sequence analyses members indicate distinct within family. Computer searches databases have revealed similarity Tec ORF1s previously described Tc1-IS630 transposases includes bacterial, nematode insect transposons.